Biochemical Studies on Inositol
نویسنده
چکیده
In a previous publication (1, Paper V of this series) the purification and properties of the inositol-cleaving enzyme were described. It was shown that a highly purified enzyme was obtained from rat kidneys which cleaved inositol to D-ghCUI’OniC acid. The purified enzyme was shown to possess SH groups and 1 gram atom of iron per mole of enzyme. Both the SH groups and the iron are necessary for catalytic action. This report concerns itself with further studies of the mechanism of action of this enzyme. Data are presented on the site of cleavage of inositol,l the origin of the oxygen atom which appears in the carboxyl group of glucuronic acid, and the interaction of the enzyme with a number of substances related to inosito1.
منابع مشابه
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